The American director Rob Reiner went to see Judas Priest in concert as part of his preparation for making the film This Is Spinal Tap (1984), which spoofs British heavy metal bands.
The drama-comedy film Rock Star (2001), starring Mark Wahlberg, is loosely based on the story of how Tim "Ripper" Owens replaced Rob Halford as the vocalist in Judas Priest.
The Judas Priest song "Electric Eye" was used in the temp score for Toy Story 3 (2010), but was ultimately replaced by another piece of music.
On 5 January 2014, the band appeared in the episode "Steal This Episode" (which the name itself being a parody of the System of a Down album, Steal This Album!) of comedy cartoon show The Simpsons playing a parody of their song “Breaking the Law". Their music was referred to as "death metal", for which the producers subsequently apologised by having Bart Simpson write "Judas Priest is not 'Death Metal'" in the opening sequence chalkboard gag.
One of the two main "gangs" in the remote Northern Territory community of Wadeye during the 2000s and 2010s is called the "Judas Priests". Their main enemy is known as the "Evil Warriors", also the title of a 1985 song by death metal band Possessed.