Live performances and covers
Madonna and her dancers emerge from behind the moving screens for the performance of "4 Minutes" on the Sticky & Sweet Tour
The song was performed during the Hard Candy Promo Tour and Sticky & Sweet Tour (2008–09). At the promotional tour, "4 Minutes" was performed as the third song of the setlist. Madonna wore a shiny black outfit with black tails, Adidas track pants and high-heeled, lace-up boots for the performance. Justin Timberlake made an appearance alongside Madonna, at the Roseland Ballroom in New York, to perform the song. As Timbaland appeared on the video screens, the beat of the song started. The four side-stage video screens began to glide across the stage, and swiveled around to reveal Timberlake behind one and Madonna behind the other. They performed the song in a similar choreography from the music video.
During the "4 Minutes" performance on the Sticky & Sweet Tour, Madonna wore a futuristic robotic outfit designed by Heatherette. She coupled it with metallic plates on her shoulder and a wig with long curled hair. Madonna and her dancers emerged from behind backdrops, on which Timbaland and Timberlake appeared, to perform their lines. An apparent duet between Madonna and Timberlake ensues, with Timberlake singing and dancing his part from the screens. He joined Madonna in person, for the show at Los Angeles's Dodger Stadium on November 6, 2008, the same show in which Britney Spears appeared alongside Madonna to perform "Human Nature". They performed "4 Minutes" in similar fashion to the promotional tour choreography. Timbaland sang his part of the song in person on November 26, 2008 at Dolphin Stadium in Miami Gardens, Florida. "4 Minutes" was also used as mashups during the performance of songs like "Vogue" and "Hung Up". On July 27, 2017, Madonna made a special appearance at Leonardo DiCaprio's annual fundraising gala, which took place on Saint-Tropez, France, and performed "4 Minutes" dressed in a green suit with feathers.
"4 Minutes" was used in the film Get Smart (2008), in a scene and its film credits. It was one of the songs covered by the cast of Glee during the April 20, 2010 episode "The Power of Madonna". The fictional character Kurt Hummel, portrayed by Chris Colfer, sang Madonna's parts while Mercedes Jones (Amber Riley) sang Timberlake's. In the episode, the song is performed during a routine by the high-school cheering squad, accompanied by the school band. The version was released both as a digital download single and on the EP, Glee: The Music, The Power of Madonna. The "4 Minutes" cover charted on the Hot Digital Songs of Billboard at number 55 on May 8, 2010, while reaching number 89 and number 70 on the Billboard Hot 100 and Canadian Hot 100, respectively.