As an instrumental piece, "Fuel" was the theme song and used as background music during race recaps for NASCAR broadcasts on NBC and TNT from 2001 to 2003 seasons. (It was also used for the 2004 coverage of the Daytona 500, but not as the theme song.) However, from the MBNA Cal Ripken Jr. 400 until the end of the 2001 season, the opening scream ("Give me fuel, Give me fire, Give me that which I desire!") was removed because of its close association with terrorists in the wake of the September 11 attacks. The pre-release version of the song entitled "Fuel for Fire" (recorded on October 5, 1995) (with different lyrics) appears on the NASCAR Full Throttle CD. Sometimes at live concerts, James and Kirk slide their guitars to make an engine roaring sound to add the taste of the theme of the song. The song was used as the entrance music for NASCAR drivers Kyle Busch and Joey Logano when they guest-starred on the October 26, 2009 episode of WWE Raw.
The song was featured in the television series House M.D., on the episode "Known Unknowns".
Avril Lavigne has covered the song live paying tribute to the band at MTV Icon: Metallica, held in Universal City, California in 2003.
The song was featured in the video game Test Drive Off-Road Wide Open as the game's opening video music and a selectable music track in the game.
The song was also featured as the theme song for Hot Wheels Turbo Racing.
The song was featured in the 2009 music game Guitar Hero: Metallica as a playable track.
"Fuel" was used in a 2016 television advertising campaign for the 2016 Dodge Charger and Challenger.
The opening verse ("Gimme Fuel, Gimme Fire" etc.) is also used, following a short section of ELPs Fanfare for the Common Man, as introduction music to Top Fuel Dragster class racing at Santa Pod raceway England.
"Fuel" is also used as the intro song for the Indy Fuel hockey team.